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KMID : 0604520200460010067
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Journal of the Society of Cosmetic Scientists of Korea
2020 Volume.46 No. 1 p.67 ~ p.72
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Inhibitory Effect of Lonicera japonica Extract on MMP-1 Production in Human Dermal Fibroblast
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Jeong Yeon-Su
Lee Jin-Young
Ko Jae-Young
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Abstract
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In this study, we attempted to obtain an active ingredient that inhibits the production of matrix metalloproteinase-1 (MMP-1) that breaks down collagen in human skin fibroblasts. More than 50 plant extracts were screened, and Lonicera japonica was selected for this study. The stem of L. japonica was extracted with 70% ethanol and fractions with solvents in the order of hexane, ethyl acetate, and butanol. MMP-1 production were significantly inhibited at the concentration of 50 μg/mL of the ethyl acetate layer and 200 μg/mL of the butanol layer. To get a fraction containing all of these effective components, 80% ethanol fraction (LJ F80) was obtained through HP20 resin column chromatography. The reference substance, loganin and LJ F80 inhibited dose-dependently MMP-1 production.
At the same concentration, LJ F80 showed a higher inhibitory effect than loganin. The stability of this fraction was analyzed with HPLC while kept storing at 4 ℃, room temperature, and 40 ℃, for 16 week. The stability was maintained as ± 10% of initial value with reference loganin. Therefore, it is thought that LJ F80 of L. japonica may be used to improve wrinkles.
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KEYWORD
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Lonicera japonica, Matrix metalloproteinase-1, Loganin, Stability, Anti-wrinkle
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